Rapid Production of Lilium auratum Bulbs from Zygotic Embryos
Takuo Furyuya1,3 and Koji Nomura1,2*
1Masters Programs of Biosystem Studies, University of Tsukuba, Tsukuba, Ibaraki 305-8572
2Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, Ibaraki 305-8572
3Present address: Ibaraki Prefectural Sashima Senior High School, Shasima, Ibaraki 306-0501
*Author for Correspondence.
Institute of Agriculture and forestry, University of Tsukuba, Ibaraki 305-8572.
Tel and Fax: +81-29-8536692;
Email: daucus@sakura.cc.tsukuba.ac.jp
Key words: Lilium auratum Lindl, ovule culture, embryo culture, propagation, genetic diversity
Abstract.
An in vitro culture system for Lilium auratum Lindl. zygotic embryos was established to accelerate the development
of bulblets. High rates of germination were observed on 50 days after pollination (DAP) or later, while the rate of normal
growth increased after 70 DAP. Peeling of the seed coats was necessary for rapid in vitro germination, indicating inhibition by the
seed coats. We found that embryos obtained from 90 DAP capsules were the best materials for suppressing dormancy. Further
development of bulblets was accelerated by culturing them in the presence of 9 % sucrose. In this culture system, the period
for development of mature bulblets was reduced from a couple of years to one year after pollination.
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