Asnita Abu Harirah and Norzulaani Khalid
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
*Author for Correspondence.
Institute of Biological Sciences,
Faculty of Science,
University of Malaya,
50603 Kuala Lumpur, Malaysia.
Regenerated plants were established using male inflorescence of Musa acuminata cv. Berangan. Explants were
cultured on Murashige and Skoog (MS) solid medium supplemented with three different concentrations of 6-
benzylaminopurine (BAP). After 2 weeks, whitish bud-like structures (WBLS) were obtained and MS media supplemented
with 70.0 µM BAP was observed as the best media for the growth of WBLS. After 3 months, shoot-like structures emerged
and MS media supplemented with 31.0 µM BAP gave a large number of shoot formation. Normal looking plantlets were
obtained within 4 - 6 months with an average of 80 - 130 shoots regenerated from each male inflorescence. Random
amplified polymorphic DNA (RAPD) was carried out to determine the clonal fidelity on in vitro Musa acuminata cv. Berangan
micropropagated from male inflorescence derived from the same mother plant. Eighteen arbitrary decamer primers were used
to amplify DNA from in vitro plants materials. All RAPD profiles from regenerated plants were monomorphic thus no
somaclonal variation was detected. A total of 81 bands were scored from PCR amplification of genomic DNA from 15
micropropagated plants. This was further confirmed by the value of similarity index which was equaled to 1. This result
implied that male inflorescence could be used as an alternative explant for commercial planting.