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As. Pac. J. Mol. Biol. & Biotech., June 2006 Vol. 14, 27-31

The Operation Performance of an Expanded Bed Contactor Characterised by Mechanical Stirring

Tau Chuan Ling1,2* , John Chi Wei Lan1,3 and Andrew Lyddiatt1,4

1Biochemical Recovery Group, Department of Chemical Engineering, School of Engineering,
University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
2Department of Process and Food Engineering, Faculty of Engineering,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
3Biomass Laboratory, Energy and Resources Laboratories,
Industrial Technology Research Institute, Hsin-Chu (310), Taiwan.
4Millipore Biopharmaceutical Division, Bioprocessing Ltd, Medomsley Road, Consett DH8 6SZ, United Kingdom.

*Author for Correspondence.
Department of Process and Food Engineering,
Faculty of Engineering,
Universiti Putra Malaysia,
43400 UPM, Serdang, Selangor Malaysia.
Tel: +603 - 89466360
Fax: +603 - 86567123
Email: ltc555@eng.upm.edu.my

Abstract.
The performance of an expanded bed contactor (UpFront i.d. 20 mm) characterised by mechanical stirring flow distribution in the adsorption of intracellular proteins from concentrated unclarified yeast extract was investigated. High density pellicular adsorbent (UpFront steel-agarose; ρ = 2.65 g ml-1) derivatized with selective ligand chemistries (Cibacron Blue 3GA) was adopted in this study. The adsorption of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) from bakersí yeast was chosen as a demonstration of this approach. It was demonstrated that a high biomass throughput adsorption operation (25 % ww/v of yeast extract) was achieved in this contactor design.

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