ÿþ<!doctype html public "-//w3c//dtd html 3.2//en"> <html> <head> <title>APJMBB Volume 14 Number 2, Dec 2006</title> <meta name="GENERATOR" content="Arachnophilia 4.0"> <meta name="FORMATTER" content="Arachnophilia 4.0"> </head> <STYLE TYPE="text/css"> .head { font-family: Arial narrow, Times new roman, Verdana, Helvetica, sans-serif; font-size: 11pt; color: #000000; font-style: italic;} .title { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 14pt; color: #000000; font-weight: bold;} .text { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 12pt; color: #000000;} .affil { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 11pt; color: #000000; font-style: italic;} .sechead { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 12pt; color: #000000; font-weight: bold;} A {text-decoration:none} A:hover {color:#ff8040;} .text3 { font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 9pt; color: #000000;} .text4 { font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 9pt; color: #0000ff;} .text2 { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 9pt; color: #000000;} </STYLE> <body bgcolor="#ffffff" text="#000000" link="#0000ff" vlink="#800080" alink="#ff0000"> <img src="../images/apjlogos.gif" width="125" height="87" alt=""><br><hr width=90% align=left> <span class=text3><a href="142cont.htm">[ Contents ]</a> <a href="142b.htm">[ Previous abstract ]</a> [ Next abstract ] <a href="../html141/141cont.htm">[ Previous issue ]</a> [ Next issue ] <a href="../../apjhome.htm">[ APJMBB home ]</a></span><hr width=90% align=left> <p class=head>As. Pac. J. Mol. Biol. &amp; Biotech., Dec 2006 Vol. 14, 47-55</p> <table width="90%" border="0" cellspacing="0" cellpadding="5"> <tr> <td width=100% valign=top><span class=title>Analysis of tissue culture-derived regenerants using methylation sensitive AFLP </span><br> <br> <span class=text>Rajinder Singh*, Cheong Peck Lei, Koh Shu Jiun, and Cheah Suan Choo</span><br> <br> <span class=affil>Malaysia Palm Oil Board (MPOB), Headquarters, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor.</span> <p class=text2><sup>*</sup>Author for Correspondence.<br> Advanced Biotechnology & Breeding Centre<br>Malaysia Palm Oil Board (MPOB) Headquarters,<br>6 Persiaran Institusi Bandar Baru Bangi<br>43000 Kajang,Selangor.<br>Tel: 03-87694501<br>Fax: 03-89261995<br> Email: rajinder@mpob.gov.my </p> <p class=text align=justify><span class=sechead>Abstract.</span><br> Tissue culture-derived plants of oil palm (<i>Elaeis guineensis</i>) can develop abnormal flowers in which stamen primordia are converted into carpel-like tissues. This often gives rise to abnormal fruits which are said to be  mantled . Individual palms showing variation in mantling and reversion to the normal phenotype over time have been observed. Four ortets and their individual ramets were compared using methylation-sensitive amplification polymorphism (MSAP). Polymorphisms were observed with all methylation-sensitive restriction endonucleases with <i>Hpa</i>II giving the highest number of polymorphic bands (0.62%) followed by <i>Msp</i>I (0.48%) and <i>Pst</i>I (0.19%). Polymorphisms detected were associated with both the mantled characteristic and tissue culture changes. Comparison between <i>Hpa</i>II and <i>Msp</i>I generated banding patterns showed the occurrence of hypomethylation, hypermethylation, hemi-methylation and change of methylation site, suggesting that the mantling phenotype was not caused by loss of methylation only. In fact, the occurrence of hypermethylation (46.7%) in this study is higher than hypomethylation (32.7%). From the results, it was also clear that the mantled abnormality was not caused by major changes in the genome during tissue culture. The mantled phenomenon was most likely associated with changes in methylation of specific loci. However, no consistent polymorphism was observed between the different sets of normal and abnormal ramets. This suggests that, whilst this method is an effective way of detecting variation in tissue culture-derived plants, it cannot identify the causal basis of the mantled fruit abnormality. </p> <a href="1424755.pdf" class=text4>[Get pdf]</a> </td></tr></table> <br> <br> <br> <br> </body> </html>