ÿþ<!doctype html public "-//w3c//dtd html 3.2//en"> <html> <head> <title>APJMBB Volume 15 Number 3, Oct 2007</title> <meta name="GENERATOR" content="Arachnophilia 4.0"> <meta name="FORMATTER" content="Arachnophilia 4.0"> </head> <STYLE TYPE="text/css"> .head { font-family: Arial narrow, Times new roman, Verdana, Helvetica, sans-serif; font-size: 11pt; color: #000000; font-style: italic;} .title { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 14pt; color: #000000; font-weight: bold;} .text { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 12pt; color: #000000;} .affil { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 11pt; color: #000000; font-style: italic;} .sechead { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 12pt; color: #000000; font-weight: bold;} A {text-decoration:none} A:hover {color:#ff8040;} .text3 { font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 9pt; color: #000000;} .text4 { font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 9pt; color: #0000ff;} .text2 { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 9pt; color: #000000;} </STYLE> <body bgcolor="#ffffff" text="#000000" link="#0000ff" vlink="#800080" alink="#ff0000"> <img src="../images/apjlogos.gif" width="125" height="87" alt=""><br><hr width=90% align=left> <span class=text3><a href="153cont.htm">[ Contents ]</a> <a href="153a.htm">[ Previous abstract ]</a> <a href="153c.htm">[ Next abstract ]</a> <a href="../html152/152cont.htm">[ Previous issue ]</a> [ Next issue ] [ <a href="../../apjhome.htm">APJMBB home</a> ]</span><hr width=90% align=left> <p class=head>As. Pac. J. Mol. Biol. &amp; Biotech., Oct 2007 Vol. 15, 107-113</p> <table width="90%" border="0" cellspacing="0" cellpadding="5"> <tr> <td width=100% valign=top><span class=title>Computational prediction of microRNAs from Oil Palm (<i><b>Elaeis guineensis</b></i> Jacq.)expressed sequence tags </span><br> <br> <span class=text>Nadirah Md Nasaruddin<sup>1</sup>, K.Harikrishna<sup>1</sup>, Rofina Yasmin Othman<sup>2</sup>,Lim Saw Hoon<sup>3</sup> and Jennifer Ann Harikrishna<sup>2</sup>*</span><br> <br> <span class=affil><sup>1</sup>Sime Darby Technology Centre Sdn Bhd, 2 Jalan Tandang, 46050 Petaling Jaya, Selangor<br> <sup>2</sup>Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur<br> <sup>3</sup>Biotechnology, Malaysia University of Science and Technology, Block C Kelana Square, 47301 Petaling Jaya, Selangor </span> <p class=text2>*Author for Correspondence.<br> Genetics and Molecular Biology,<br>Institute of Bio­logical Sciences, <br>Faculty of Science, University of Malaya, <br>50603 Kuala Lumpur, Malaysia. <br>Tel: (603)79675896; Fax: (603)79675908; <br>Email: jennihari@um.edu.my </p> <p class=text align=justify><span class=sechead>Abstract.</span><br> MicroRNAs (miRNAs) are defined as &asymp;22nt non-coding RNAs derived from one arm of longer hairpin stem-loop precursors through the action of Dicer, that are capable of silencing gene activities at the transcriptional or post-transcriptional level. miRNA plays important regulatory roles in animal and plant development and as such the identification of miRNA en­coding regions of genomes is an important part of gene annotation and functional genomics. A total of 7,284 <i>Elaeis guineensis</i> Jacq. expressed sequence tags (EST) were compared to previously known <i>Arabidopsis thaliana</i> and <i>Oryza sativa</i> miRNA, to search for novel miRNA from oil palm. Twenty-five EST contigs with homology to previously known miRNAs were identified. After predicting the RNA secondary structure, five new potential miRNA were identified in oil palm. One of the most promising potential miRNA was miR160 which has very strong characteristics of an miRNA precursor structure and 100% identity with the mature miR160 from other plant species. </p> <a href="153b.pdf" class=text4>[Get pdf]</a> </td></tr></table> <br> <br> <br> <br> </body> </html>