Development of simple sequence repeat (SSR) markers for
oil palm and their application in genetic mapping
and fingerprinting of tissue culture clones
Rajinder Singh1*, Jayanthi Nagappan1, Soon-Guan Tan2,
Jothi Malar Panandam3 and Suan-Choo Cheah1,4
1Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB),
P.O. Box 10620, 50720 Kuala Lumpur
2 Biology Department, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang
3 Department of Animal Sciences, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang
4 Present address: Asiatic Centre for Genome Technology, Lot L3-I-1, Enterprise 4,
Technology Park Malaysia, 57000 Kuala Lumpur
*Author for Correspondence.
Advanced Biotechnology and Breeding Centre,
Malaysian Palm Oil Board (MPOB),
P.O. Box 10620, 50720 Kuala Lumpur.
Tel: 603-8769 4501; Fax: 603-8925 1996;
Email: rajinder@mpob.gov.my
Abstract.
This study describes the application of a simple and effective method to isolate SSR markers from oil palm genomic sequences. A total of 12 informative SSR markers are described. The SSR markers were found suitable for genome analysis and DNA fingerprinting of oil palm tissue culture clones. Eleven of the 12 SSR markers exhibited expected Mendelian segregation ratios when tested on a mapping population, indicating their suitability for genetic mapping studies. The markers identified in the species Elaeis oleifera also showed applicability in a second species, that is Elaeis guineensis. Apart from genetic mapping, the SSR markers also showed promise as molecular probes for DNA fingerprinting of oil palm tissue culture clones. The SSR markers can be used for clonal identification, monitoring line uniformity between and within clones and detecting culture mix-up.
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