ÿþ<!doctype html public "-//w3c//dtd html 3.2//en"> <html> <head> <title>APJMBB Volume 15 Number 3, Oct 2007</title> <meta name="GENERATOR" content="Arachnophilia 4.0"> <meta name="FORMATTER" content="Arachnophilia 4.0"> </head> <STYLE TYPE="text/css"> .head { font-family: Arial narrow, Times new roman, Verdana, Helvetica, sans-serif; font-size: 11pt; color: #000000; font-style: italic;} .title { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 14pt; color: #000000; font-weight: bold;} .text { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 12pt; color: #000000;} .affil { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 11pt; color: #000000; font-style: italic;} .sechead { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 12pt; color: #000000; font-weight: bold;} A {text-decoration:none} A:hover {color:#ff8040;} .text3 { font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 9pt; color: #000000;} .text4 { font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 9pt; color: #0000ff;} .text2 { font-family: Times new roman, Verdana, Arial, Helvetica, sans-serif; font-size: 9pt; color: #000000;} </STYLE> <body bgcolor="#ffffff" text="#000000" link="#0000ff" vlink="#800080" alink="#ff0000"> <img src="../images/apjlogos.gif" width="125" height="87" alt=""><br><hr width=90% align=left> <span class=text3><a href="153cont.htm">[ Contents ]</a> <a href="153f.htm">[ Previous abstract ]</a> [ Next abstract ] <a href="../html152/152cont.htm">[ Previous issue ]</a> [ Next issue ] [ <a href="../../apjhome.htm">APJMBB home</a> ]</span><hr width=90% align=left> <p class=head>As. Pac. J. Mol. Biol. &amp; Biotech., Oct 2007 Vol. 15, 153-156</p> <table width="90%" border="0" cellspacing="0" cellpadding="5"> <tr> <td width=100% valign=top><span class=title>Rapid isolation method of soil bacilli and screening oftheir quorum quenching activity </span><br> <br> <span class=text> Kok-Gan Chan*, Siew-Zhen Tiew and Ching-Ching Ng </span><br> <br> <span class=affil> Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Malaysia. </span> <p class=text2>*Author for Correspondence.<br> Institute of Biological Sciences (Genetics & Molecular Biology), <br>Faculty of Science, University of Malaya, <br>50603 Kuala Lumpur. <br>Tel: 603-79675162; <br>Email:kokgan@um.edu.my</p> <p class=text align=justify><span class=sechead>Abstract.</span><br> In Gram-negative bacteria, quorum sensing is mediated by <i>N</i>-acylhomoserine lactones (AHL) to regulate different biological functions, including production of virulence factors. Quorum quenching refers to the interruption of quorum sens­ing, and the most attractive way is to degrade the AHL molecules. With the aim of isolating soil bacteria capable of blocking quorum sensing by inactivating AHL, a novel enrichment method has been designed. Bacteria degrading AHL were isolated from a Malaysian soil sample. We identified twelve isolates which possessed an <i>aiiA</i> homologue gene by using PCR approach and three strains were selected for further analysis. AHL-inactivation assay showed that these three isolates rapidly degraded synthetic 3-oxo-C6-HSL <i>in vitro</i>. Morphological phenotypes and 16S ribosomal DNA sequence analysis indicated that these isolates fell into the genus of <i>Bacillus</i>. Our data suggest that soil <i>Bacillus</i> spp. can be isolated using the selection method and a number of soil bacilli in Malaysian soil exhibited quorum quenching activity.</p> <a href="153g.pdf" class=text4>[Get pdf]</a> </td></tr></table> <br> <br> <br> <br> </body> </html>