*Author for Correspondence.
Mailing address: Regional Plant Resource Centre,
Bhubaneswar –751 015, India.
Email: nguc2003@yahoo.co.in

Abstract.
208 fungi isolated from mangrove ecosystem of Bhitarkanika were screened for L asparaginase activity. Among them 85 fungi exhibited enzyme activity in biomass and 20 fungal strains showed L asparaginase activity in culture filtrate . Penicillium sp. strain PF 52 and RF 22 were found to be good source of L asparaginase as they exhibited highest activity in biomass and culture filtrate respectively. During screening programe, Scopulariopsis strain FMG 133 and single unidentified fungal strain FMG 181 showed good enzyme activity i.e. 10.28 & 7.85 i. u. /ml/g activity in biomass and 6.25 & 11.44 i.u./ml/g in the well as culture filtrate of 10 day old culture. Over all Aspergillus, Penicillum, Fusarium, Helminthosporium , Scophulariopsis , Paecilomyces and Pestalotiopsis were found to be good source of L asparaginase enzymes in this study.