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As. Pac. J. Mol. Biol. & Biotech., January 2012 Vol. 20, issue 4, 25-31

Amplification and sequencing of partial-length disease resistance gene homologues coding for NBS-LRR proteins in oil palm (Elaeis guineensis)

Chin Chiew Foan1*, Y.W. Lee2, Joon S. Tan3 and Sharifah Shahrul Rabiah Syed Alwee3

1University of Nottingham Malaysia Campus, School of Biosciences, Faculty of Science, Jalan Broga,43500 Semenyih, Selangor Darul Ehsan, Malaysia;
2ACGT Sdn Bhd, Lot L3-l-1, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000 Kuala Lumpur, Malaysia;
3FELDA Agricultural Services Sdn Bhd, Tingkat 7, Balai Felda, Jalan Gurney 1, 54000 Kuala Lumpur, Malaysia.

*Author for Correspondence.
Chin Chiew Foan,
University of Nottingham Malaysia Campus,
School of Biosciences,
Faculty of Science,
Jalan Broga, 43500 Semenyih,
Selangor Darul Ehsan,
Email - Chiew-Foan.Chin@nottingham.edu.my.

One of the key approaches for disease prevention in oil palm is to develop a disease resistant variety; this can be achieved through tagging of resistance genes in oil palm. In this study, a total of three putative resistance gene homologues (RGHs) have been isolated from oil palm (Elaeis guineensis) using primers targeted at the conserved motifs of the nucleotide binding site (NBS) domain. Comparison of the isolated RGH sequences with the NCBI GenBank database showed that one of the homologues had high identity to the disease resistance like proteins in coconut (90%) and tomato (87%). Phylogenetic analysis indicated that the RGHs clustered with R genes found in Arabidopsis. The findings will be useful for studying of resistant genes in oil palm.

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