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As. Pac. J. Mol. Biol. & Biotech., Apr 2015 Vol. 2, 36-47

Isolation and characterization of a biodegrading 3-chloropropionic acid Burkholderia cepacia WH1 isolated from abandoned agricultural land

Wafaa Hassan Muslem1,3, Fahrul Huyop3*, Iffah Izzati Zakaria4 and Roswanira Abdul Wahab2*

1Department of Biology, College of Science, Al-Mustansiriyah University, Iraq,
2Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia,
3Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia,
4Iffah Izzati Zakaria, Natural Products and Drug Discovery Center, Malaysian Institute of Pharmaceuticals and Nutraceuticals, National Institutes of Biotechnology Malaysia, Ministry of Science, Technology and Innovation, Block 5-A, Halaman Bukit Gambir, 11700 Pulau Pinang, Malaysia.

*Author for Correspondence.
Roswanira Abdul Wahab,
Department of Chemistry,
Faculty of Science,
Universiti Teknologi Malaysia,
81310 UTM Johor Bahru,
Johor,
Malaysia.
Tel.: +607 5534148,
Fax: +607 5566162
Email: roswanira@kimia.fs.utm.my

and

Fahrul Huyop,
Department of Biosience,
Faculty of Biosciences and Medical Engineering,
Universiti Teknologi Malaysia,
81310 UTM Johor Bahru,
Malaysia.
Tel.: +607 5534163,
Fax: +607 5566162,
Email: fzhutm@gmail.com

Abstract.

The widespread use of herbicides that may contain the recalcitrant halogenated compound 3-chloropropionic acid poses significant environmental hazards and may prove detrimental to human kind. Therefore, it is important that an environmentally friendly bio-based method to detoxify such substance is developed. Consequently, the research detailed here investigated the isolation and identification of a bacterial strain that could degrade 3-chloropropionic acid (3-CP) as its sole carbon source. A dehalogenase producing bacteria capable of utilizing 3-CP was successfully isolated from abandoned agricultural land and designated strain WH1. Analysis of 16s rRNA as well as biochemical and morphological tests found that the strain WH1 showed a 96% sequence identity with and characteristics similar to that of Burkholderia cepacia. Analysis of phylogeny and BIOLOG confirmed that the strain WH1 was indeed B. cepacia. The bacteria grew well at 37°C in media containing 10 mM 3‒CP but exhibited a rather slow doubling time of 43.62 h, with an optimum chloride ion release of 0.194 µmol Cl/mL. Most importantly, analysis by high perfomance chromatography revealed that the B. cepacia isolate effectively degraded ~100% of available 10 mM 3CP. This is the first report detailing a B. cepacia strain able to competently utilize 3-CP as its sole carbon source.

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