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As. Pac. J. Mol. Biol. & Biotech., Apr 2015 Vol. 2, 48-58

Growth of Moraxella osloensis COK1, a Novel Strain of Bacteria Isolated from Subbituminous Coal, in Dibenzothiophene and Coal Medium

Megga Ratnasari Pikoli1*, Pingkan Aditiawati2, Dea Indriati Astuti2 and Akhmaloka3

1Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri (UIN) Syarif Hidayatullah Jakarta, Jl. Ir. H. Juanda 95, Ciputat, Tangerang, Banten 15412, Indonesia,
2Microbial Biotechnology Research Group, School of Life Science and Technology, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung,Jawa Barat 40132, Indonesia,
3Biochemistry Research Group, Faculty of Mathematics and Natural Science, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung, Jawa Barat 40132, Indonesia.

*Author for Correspondence.
Megga Ratnasari Pikoli,
Department of Biology,
Faculty of Science and Technology,
Universitas Islam Negeri (UIN) Syarif Hidayatullah Jakarta,
Jl. Ir. H. Juanda 95,
Ciputat, Tangerang,
Banten 15412,
Indonesia.
Tel.: +62217401925
Email: meggapikoli@uinjkt.ac.id and meggapikoli@yahoo.com


Abstract.

Bacteria capable of desulfurization of organic substances in coal and petroleum are required to improve the quality of the fuel. We have thus isolated bacteria from coal sources and examined their growth and desulfurization activities in monocultures. Three viable strains of bacteria were obtained using two procedures: one-step enrichment and gradual enrichment. A medium was used which was enriched with dibenzothiophene, the most dominant organic sulfur in coal. Based on analysis of 16S rRNA gene, it was found that the bacteria were closely related to Moraxella osloensis, Pseudomonas aeruginosa, and Enhydrobacter aerosaccus. The evolutionary relationships amongst these bacteria were analyzed by phylogenetic inference and a phylogenetic tree constructed. The growth of the viable bacteria were observed independently in medium containing dibenzothiophene and coal. Moraxella osloensis has not been previously reported as a bacteria associated with coal, or in using organic sulfur in coal. However this study demonstrated that the most suitable medium for growth was a coal medium, indicating that the isolates may potentially be developed as a monoculture for coal desulfurization. The other two isolates showed unsteady growth, and further study is needed to enable their growth in mixed culture and to meet the requisites of their natural interactions.

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