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As. Pac. J. Mol. Biol. & Biotech., Dec 1998 Vol. 6(2)

pg.79 Construction of a single chain variable fragment (ScFv) antibody recognizing the dengue 2 virus envelope protein
Hui Yee Chee and Sazaly AbuBakar*
Department of Medical Microbiology, Universiti Malaya Medical Center
Kuala Lumpur, Malaysia

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pg.89

Enhancement of the immunogenicity of Mycobacteyium leprae peptides by means of polymerization
Alexei F. Licea1,4 , Maria C. Gutierrez2,4, Jose Esparza3, Sergio Estrada Parra3, Iris Estrada G3,
Fausto Quesada Pascual3 and Lourival D. Possani4*
1Facultad de Ciencias Quimicas, Universidad Autonoma de Baja California. Calzada Tecnologico s/n Mesa de Otay.
Tijuana B. C. 22390, Mexico; 2Centro de Biotecnologia, Universidad Autonoma de Morelos, Av. Universidad s/n
Cuernavaca, Mor 62210 Mexico; 3Departamento de Inmunologia, Escuela Nacional de Ciencias Biologicas, Instituto.
Politecnico Nacional, Prolongacion de Carpio y Plan de Ayala, Mexico RE 11340 Mexico; 4Departamento de.
Reconorimiento Molecular y Bioestructura, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico.
Apartado Postal 510 3, Cuernavaca, Mor 622 71 Mexico.

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pg.97 Regulation of the aprE (Subtilisin) gene in abrB mutants of Bacillus subtilis
Jorge Olmos 1*, Alejandro Sanchez1 and Ramon DeAnda2
1Molecular Microbiology Laboratory. Centro de Investigacion Cientifica y de Educacion Superior de Ensenada (CICESE),
Ensenada, Mexico.
2Molecular Microbiology Department, Instituto de Bioteenologia, UNAM. Cuernavaca, Mexico

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pg.105 Mn 2+ Activates Multi Step DNA Cleavage in Rat Liver Nuclei
Salmaan H. Inayat Hussain*, Gerald A Cohen' and Kelvin Cain'
Department of Biomedical Science, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda
Abdul Aziz, 50300 Kuala Lumpur, Malaysia. 'MRC Toxicology Unit, Centre for Mechanisms of Human Toxicity,
Hodgkin Building, PO Box 138, Lancaster Rd., Leicester, LE1 9HN, UK.
>
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pg. 115 Factor VIII gene deletion in a Thai hemophilia A patient
P. Pung amritt', A. Treesucon', S. Petrarat', G. Veerakul,
C. Mahasandana' and P.T. Yenchitsornanus'*

'Hematology Unit, Department of Pediatrics, 'Division of Medical Genetics, Department of Medicine, 'Molecular Genetic
and Medical Molecular Biology Units, Office for Research and Development,
Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand

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pg. 121 Allele frequency distribution of the VNTR locus d1s80 and the genetic relationship of Filipinos to other populations
Sheila R. Alcantara' and Saturnina C. Halos'*
'National Institute of Molecular Biology and Biotechnology, College of Science, University of the Philippines
Diliman, Quezon City 1101
2 Biology Department, College of Science, De La Salle University, Taft Avenue,
Manila, Philippines

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pg. 131 Properties of four cucumber mosaic cucumovirus isolates from West of Malaysia
O.M. EI Sanousi1, C.A. Ong2, K Yusoff1, S. Napis3 and N. Abdul Sarnad1*
1Dept. of Biochemistry and Microbiology, 3Dept. of Biotechnology,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
2MARDI Serdang, G.P. 0. Box 12301, 50 774 Kuala Lumpur, Malaysia

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pg. 139 Nitrogen metabolism of aluminum tolerant peanut cell suspension cultures under aluminum stress
M. A. Syed*, M. Marziah and A.M. Puad
Department of Biochemistry and Microbiology, Universiti Putra Malaysia, 43400 Serdang, Selangor D.E.

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pg. 145 Isolation and transient transformation of stem and leaveprotoplasts from sweet potato (Ipomoea batatas L.)
Garcia, R., Pimentel, E., Somonte, D., Mena, J., Zaldua, Z., Lopez, A., Moran, R., and Garcia*, M.
Centro de lngenieria Genetica y Biotecnologia. P.O. Box 387, C.P. 70100. Camaguey, CUBA

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pg. 153 Properties of Bifidobacterium spp.: antibacterial, growth in milk and survival during low temperature storage
A.M. Yazid1*, A.M. Ali2, V. Kalaivani1 and M. Shuhaimi1
1Department of Food Technology, 2Department of Biotechnology, Faculty of Food Science and
Biotechnology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

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pg. 161 Effect of initial carbon and nitrogen sources concentrations on growth of Aspergillus awamori and glucoamylase production
A.B. Ariff1* and C. Webb2
1Department of Biotechnology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia,
2Department of Chemical Engineering, UMIST, P 0 Box 88, Manchester M60 IQD, United Kingdom

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pg. 171 Stability enhancement of protease by immobilization
A Chellapandian1 and C.A. Sastry2*
1Department of Bioengineering, Institute of Biotechnology, UNAM,
Apdo 510 3 Cuernavaca, Morelos 62271, Mexico
2Institute of Advanced Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.

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pg. 175 A colourimetric dipstick colloidal dye immunoassay for rapid diagnosis of active cases of canine dirofilariasis
Wan Omar Abdullah*, Low Kiat Cheong1, Vivian Jacob Gomes1,
Sulaiman Osman1 and Yusof Suboh1

Department of Medical Sciences, Faculty of Medicine and Health Sciences,
Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor; 1Department of Parasitology and Medical
Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz,
50300 Kuala Lumpur

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pg.181 Characteristics of a dominant lignin peroxidase isoenzyme of Phanerochaete chrysosporium from solid state fermentation on rice husks
Darah, R. Burke and C. 0. Ibrahim
Fermentation and Enzyme Technology Laboratory, School of Biological Sciences, Universiti Sains Malaysia
11800 Minden, Penang, Malaysia

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pg.187 Decolorization of kesinai (Streblus asper) leaf extract
Y.M.A. Idris1, A.M. Yazid2 and A.B. Sipat1*
1Department of Biochemistry and Microbiology; 2Department of Food Technology,
Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor D.E., Malaysia

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pg.191 Properties of Bifidobacterium spp.: antibacterial, growth in milk and survival during low temperature storage
Sunee Kertbundit*, Pongsopee Attasart, Sakol Panyirn and Milajuricek
Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand

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pg. 199 A simple protocol for purifying recombinant bacteriophage lambda
DNA clones for automatic sequencing

J. Xia, J. Borevitz1 and R. Zhang*
Plant Molecular Ecophysiology Research Group, Department of Biological Sciences,
University of Wollongong, NSW 2522, Australia

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