As. Pac. J. Mol. Biol. & Biotech., Dec 1998 Vol. 6(2) : 191-198

Sequence comparison of coat protein and 3' non translated region of papaya ringspot virus isolates from Thailand

Sunee Kertbundit*, Pongsopee Attasart, Sakol Panyirn and Milajuricek

Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand

(Received 24 June 1998 / Accepted 12 October 1998)

Abstract.

The coat protein gene and most of the 3' non translated region of ten isolates of papaya ringspot virus (PRSV) were sequenced. Although there was up to 13% variation when these isolates were compared with published sequences of PRSV, less then 5% difference was observed among the coat proteins and the 3'non translated region (NTR) of Thai isolates indicating that all isolates have the same origin. Two isolates, propagated under laboratory conditions, showed greater homology with isolates from China and Taiwan than with the original one from Thailand.

Key Words: sequence comparison, coat protein, 3' non translated region, papaya ringspot virus