As. Pac. J. Mol. Biol. & Biotech., Dec 1999 Vol.7(1) : 85-88

DNA fingerprinting in chicken

N. N. Hasima^*,1. Azli and A. A. Rahmat

Institute of Biological Sciences, Faculty of Science,
University of Malaya, 50603 Kuala Lumpur, Malaysia

(Received 19February 1999 / Accepted 19 April 1999)

Abstract.

DNA fingerprints of chickens were successfully obtained using a non radioactive technique. The probe used was the human minisatellite probe, 33.15 and digestions of the genomic DNA were with HaeIII Hinfl and AluI. Average DNA fingerprint band sharing and band frequency between individuals with respect to the different proberestriction enzyme combinations were used to estimate average band sharing between individuals and average band frequency.

Key Words: Chicken, minisatellite, non radioactive labeling