As. Pac. J. Mol. Biol. & Biotech., Dec 1999 Vo7(1) : 95-96

Rapid extraction of plant genomic DNA suitable for restriction analysis and PCR amplification

Prasun K. Mukherjee

Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.

(Received 20 February 1999 / Accepted 7 April 1999)

Abstract.

A rapid method for plant genomic DNA extraction is described here. This method, which is a simple modification of the existing methods, requires only one centrifugation at I 1 000 g for 10 min. High molecular weight genomic DNA from various plants suitable for restriction analysis and PCR amplification has been obtained.

Key Words: DNA, plant, fungi, RFLP, RAPD