As. Pac. J. Mol. Biol. & Biotech., Dec 1999 Vol.7(2) : 173-178

Abundance of random amplified hybridising microsatellites in mungbean (Vigna radiata L.Wilczek)

S. Prasad¹, KS. Reddy² and N.Jawali^1*

¹Molecular Biology and Agriculture Division
²Nuclear Agriculture and Biotechnology Division
Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India

(Received 29 July 1999 / Accepted 10 October 1999)

Abstract.

In eukaryote genomes microsatellites are abundant and dispersed and they have gained importance as single locus markers because of their high level of polymorphism. The abundance of some tri and tetranuclcotide repeat microsatellites were surveyed in random amplified DNA fragments for the development of microsatellite markers for mungbean. Amplification products obtained from 39 arbitrary primers were screened. The data obtained on random amplified products hybridising microsatellites (RAHMs) show GGTT repeat RAHMs is the most abundant followed by CAA and CAC repeat RAHMs. The tetranucleoticle GCGT repeat RAHMs constitute nearly half as much as CAA repeat RAHMs, whilst GACA repeat RAHMs are absent. Some primer/ probe combinations were used for assessing RAHM polymorphism among 15 genotypes of mungbean. The polymorphic RAHMs (Random Amplified Microsatellite Polymorphism :RAMPO) were essentially dominant in nature (as in the case of RAPD).

Key Words: Arbitrary primers, microsatellites, mungbean, RAHM, RAMPO