As. Pac. J. Mol. Biol. & Biotech., Dec 2000 Vol. 8(2) : 161-166

Cellulose binding proteins in Ruminococcus albus D3 isolated from the rumen of a Sika deer

C.C. Sieo¹, N. Abdullah^2*, S. Jalaludin³ and YW. Ho¹

¹Institute of Bioscience, ²Department of Biochemistry and Microbiology, ³Department of Animal Science,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

Received 26 May 2000 / Accepted 14 October 2000

Abstract.

A study was carried out to detect the cellulose binding proteins (CBPs) of Ruminococcus albus D3, isolated from the rumen of a Sika deer. Buffer A supplemented with either 1% (w/v) carboxymethylcellulose (CMC or 10% (w/v) cellobiose or 5% (w/v) sodium dodecyl sulphate (SDS) was used to elute CBPs from avicel incubated with cell lysate of R. albus. Buffer A supplemented with CMC eluted two major proteins with molecular weights of 120 kDa and 100 kDa, and a few minor proteins ranging from 35 kDa to 60 kDa. Buffer A supplemented with cellobiose or SDS eluted proteins with approximate weights of 240, 120 and 100 kDa. Among the three CBPs, die 240 kDa CBP showed xylanase activity

Key Words: cellulose binding proteins, rumen bacteria, Ruminococcus albus