As. Pac. J. Mol. Biol. & Biotech., Dec 2001 Vol. 9(1) :71-74

Identification of molecular markers in sago palm (Metroxylon sagu) using polymerase chain reaction

B. Jamel, M. Morshidi and M. A. Salleh*

Molecular Biology and Biotechnology Laboratory, Faculty of Resource Science and
Technology, Universiti Malaysia Sarawak,94300 Kota Samarahan, Sarawak, Malaysia.

(Received 12 June 1998 / Accepted 12 December 2000)

Abstract.

Genomic DNA from sago palm was isolated using the cetyltrimethlammoniurn bromide (CTAB) method with some modifications. The RAPD technique, employing ten base synthetic oligonucleotides, was used to generate molecular markers for this tropical palm. Successful amplification of the sago genomic DNA was also obtained using a microsatellite sequence (GATA)4, These molecular markers will be potentially useful for genetic fingerprinting of sago palm for future breeding programmes.

Keywords: Sago palm, Molecular Markers, RAPD