As. Pac. J. Mol. Biol. & Biotech., Dec 2001 Vol 9(2) : 147-150

Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA

N. N. Hasima* ,J Saidi, K.L.Thong and A. Ansary

Institute of Biological Sciences, Faculty of Science,
University of Malaya, 50603 Kuala Lumpur, Malaysia

Received 2 August 2001 / Accepted 5 September 2001

Abstract.

Pulsed field conditions specific for Pasteurella multocida B:2 have been optimised to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200 V, ramped pulsed time of 1-25 s and a run time of 26 h. This research is important for fast and accurate characterisation of this bacterial strain following an outbreak in Malaysia.

Key Words: Pasteurella multocida, PFGE, restriction enzymes